浙江赞诚生物科技有限公司

结论体积分数为3%的百里香氛-丁香罗勒精油抗菌纸对草莓较好的保鲜效果。1001-3563(2018)19-0091-07 DOI：10.19554/j.cnki.1001-3563.2018.19.017 Effect of Antimicrobial Paper Coated with Thymus Vulgaris L. and Ocimum Gratissimum L.Essential Oil on Preservation of Strawberry LIU Guang-fa, SONG Hai-yan, LUO Wan-ru, TANG Xue-zheng, ZHANG Zheng-jian, ZHU Zi-rui (Tianjin University of Science & Technology, Tianjin 300222, China) ABSTRACT: The  work  aims  to  study  a  kind  of  antimicrobial  paper  coated  with  essential  oils  and  its  effect  on  the  preservation  of  strawberry.  Essential  oils  with  strong  antimicrobial  activity  against  4  kinds  of  microorganisms  such  as  Botrytis cinerea were screened out from 5 kinds of natural plant essential oils such as Thymus vulgaris L., and their com-ponents were identified by GC-MS. The screened essential oils were coated on paper combined with polyvinyl alcohol to prepare antimicrobial paper. The antimicrobial activity of antimicrobial paper against tested microorganisms was tested by the  method  of  fumigation,  and  the  effect  of  the  antimicrobial  paper  on  the  preservation  of  strawberry  was  studied.  The  results showed that the antimicrobial activity of Thymus vulgaris L. essential oil and Ocimum gratissimum L. essential oil against the 4 kinds of tested microorganisms was the strongest. The main components of Thymus vulgaris L. essential oil were  thymol  and  carvacrol,  and  their  peak  area  percentages  in  GC  were  77.04%  and  16.46%,  respectively.  The  main components of the essential oil of Ocimum gratissimum  L.  were  eugenol  and  β-caryophyllene,  and  their  peak  area  percentages in GC were 77.95% and 18.80%, respectively. The antimicrobial paper with essential oil concentration of 4% (volume fraction, double coating, the same below) presented the strongest antimicrobial activity, followed by antimicro-bial  paper  with  essential  oil  concentration  of  3%.  Antimicrobial  paper  with  essential  oil  concentration  of  3%  presented  the  best  effect  on  maintaining  strawberry  sensory  quality,  inhibiting  strawberry  decay  and  deferring  the  increase  of  the  total  number  of  colonies,  which  was  significantly  better  than  the  antimicrobial  paper  with  essential  oil  concentration  of  4%,  2%  and  1%  respectively  and  the  base  paper  (P<0.05).  The  effect  of  antimicrobial  paper  on  total  soluble  solids  of  strawberry  was  not  obvious.  In  conclusion,  the  antimicrobial  paper  coated  with  3%  (volume  fraction)  Thymus  vulgaris.                                                                               

2018年10月L.-Ocimum gratissimum L. essential oil has better effect on the preservation of strawberry. KEY  WORDS:  Thymus vulgaris L. essential oil; Ocimum  gratissimum  L.  essential  oil;  antimicrobial  paper;  strawberry;  preservation 食源性微生物不仅容易引起果蔬等食品的腐败，还容易引起重大公共卫生问题，如何安全有效地延长食品的货架寿命成为人们急需解决的问题。通过在食品包装材料中加入绿色安全的抗菌剂，不仅可用于控制食品的腐败，还可减少化学防腐剂的残留。

一些研究表明，百里香精油、丁香罗勒精油、孜然精油等植物精油[1—2]对一些食源性微生物具有较强的抑制作用，研究人员还对其作用机理进行了研究。申莉莉[3]发现百里香精油可使金黄色葡萄球菌的细胞壁破损，柳冬雪[4]研究发现丁香精油可破坏细菌的细胞结构。纸质材料是一种广泛使用的食品包装材料，近年来的研究表明，基于植物精油的抗菌纸可用于食品防腐保鲜领域。Rodríguez[5]使用涂布机将添加肉桂精油的石蜡乳液涂布于80  g/m2纸张上制备出了一种具有抗菌功能的活性纸，在4 °C条件下可明显降低草莓的腐烂率。

Mascheroni[6]以香芹酚为抗菌剂，蒙脱石为填料，制备涂布抗菌包装纸，该包装纸对大肠杆菌具有抑制作用。岳淑丽[7]等将肉桂精油β-环糊精包合物涂布于保鲜纸上制备出了一种抗菌纸，可延长圣女果的货架寿命。利用植物源抗菌剂制备抗菌纸的研究仅处于初步阶段，关于精油的复配、扩大抗菌纸的抗菌谱等方面未进行研究，目前也尚未见成熟的商业化产品。文中从百里香精油等5种植物精油中筛选出对灰霉等微生物具有抑制作用的精油，以筛选出的百里香精油、丁香罗勒精油为抑菌剂，将其复配并结合聚乙烯醇涂布于纸张上制得抗菌纸。研究抗菌纸对灰霉等微生物的熏蒸抑制作用，并探索抗菌纸对草莓防腐保鲜效果的影响，为天然绿色抗菌食品包装纸的研究提供技术参考。

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